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Table 1 Characteristics of the hollow cantilever and microfluidic cantilever-based platform

From: A microfluidic AFM cantilever based dispensing and aspiration platform

Parameter

Units

Cantilever

Cantilever dimensions (each leg)

Type-A: L:155 μm, W:42 μm, T:4.9 μm Type-B: L:155 μm, W:6.4 μm, T:4.9 μm

Channel dimensions (each leg)

Type-A: L:153.5 μm, W:40 μm, T: 2.2 μm Type-B: L:153.5 μm, W:3.7 μm, T: 2.2 μm

Spring constant

Type-A: 2.4 N/m (when empty); Type-B: 9.4 N/m (when empty)

Resonance frequency

Type-A: 153.94 kHz (when empty); Type-B: 110 kHz (when empty)

Aspiration and dispensing flow rate

50 fL/s @1.5 bars

External dispenser

Pressure range

0.85 bars under pressure to 5.0 bar overpressure; 0.01 bar resolution

Settling time at dispenser out

<100 ms

Climate control

Temperature range

Ambient to 40°C ±0.5

Humidity range

30% – 90% RH ±5% non condensing 90% – 100% RH ±5% condensation

Substrate alignment

 

Alignment accuracy

Between chip positions on the same substrate: <1 μm Between different chips: <2 μm