Multimodal measurement of polystyrene beads and calibration of the two imaging modes. The beads can be seen (a) in the Raman channel at 1003 cm −1, with (b) their corresponding spectrum. They can also be identified as defocused diffraction patterns in (c) the hologram, or as black dots in the corresponding (d) reconstructed amplitude image. (e) The images are then automatically registered, and shown merged with the Raman channel in red and the inverted DHM amplitude in green. Slight remaining discrepancies in position result from distortions between the two modes. Scale bars are 10 μm.