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Figure 6 | EPJ Techniques and Instrumentation

Figure 6

From: Applications of Raman micro-spectroscopy to stem cell technology: label-free molecular discrimination and monitoring cell differentiation

Figure 6

Time-course Raman spectroscopy measurements on embryoid bodies (EBs) formed by aggregation of human embryonic stem cells. (A) Schematic description of the time-course measurements. Cells were maintained on the Raman microscope for 5 days, during differentiation days 5–9. (B) Immuno-staining of control EBs grown in cardiac medium showing expression of cardiac markers α -actinin (red) and cardiac troponin I (cTnI=green) at day 7, corresponding with the onset of spontaneous beating.

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