Raman spectroscopy of cardiomyocytes derived from human embryonic stem cel006Cs. (A) Raman spectra of beating cardiomyocyte (CM) and non-cardiomyocyte (non-CM) obtained from human embryonic stem cells. PC1 represents the map corresponding to the principal component used for discrimination of CMs and non-CMs in ref . (B) Time-course mean Raman spectra of a typical beating and a non-beating EB during days 5–9 (D5–9) of differentiation. *indicates the days at which beating of the EB was observed. (C) Raman maps corresponding to PC1 scores for a beating and non-beating EB.